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. Author manuscript; available in PMC: 2012 Jun 29.
Published in final edited form as: Sci Transl Med. 2011 Jun 29;3(89):89ra57. doi: 10.1126/scitranslmed.3002156

Fig. 5. Amyloidogenic processing of APP does not vary according to human apoE isoform.

Fig. 5

(A) Representative Western blot of the proximal amyloidogenic metabolite, C99, from hippocampal homogenates (extracted with RIPA buffer) from young, sex-matched PDAPP/TRE mice. C99 was detected using 82E1 antibody. All bands were normalized to α-tubulin band intensity (n=9 mice per group; 3 to 4 months old). (B) Quantification of C99 levels after normalizing each band’s intensity to α-tubulin band intensity. (C) Quantification of β-secretase activity in hippocampal homogenates from young PDAPP/TRE mice using a sensitive FRET assay. Homogenates were incubated with fluorescent APP substrate, resulting in β-cleavage that could be followed by fluorescence increase (emission, 585 nm). The interval over which kinetics were linear was used for quantification of reaction velocity [relative fluorescence units (RFU)/min] for each sample. One-way ANOVA revealed no significant differences among groups. Values represent means ± SEM.