Skip to main content
. Author manuscript; available in PMC: 2011 Oct 13.
Published in final edited form as: J Immunol. 2011 Apr 8;186(10):5749–5757. doi: 10.4049/jimmunol.1002825

FIGURE 4.

FIGURE 4

IL-4 is necessary and sufficient to induce Eomes expression in CD8+ T cells. A, IL-4 is the key mediator for Eomes induction. Single-cell suspension of thymocytes from CIITAtg (n = 3), CIITAtgIL-4+/− (n = 2), CIITAtgIL-4−/− (n = 4), and IL-4−/− (n = 3) mice were analyzed to assess CD4/CD8 ratio (top left panels) and their expression of Eomes and PLZF in CD4 (middle left panels) and CD8 (bottom left panels) SP thymocytes. Representative data from three independent experiments (left panel) and cumulative data (right panel) are shown; bars indicate the mean ± SEM. B and C, IL-4 induces upregulation of Eomes independent of PLZF. Fetal thymuses of E15.5 B6 mice were cultured in the presence or absence of exogenous mouse rIL-4 for 7 d. Three to four fetal thymic lobes were used for each condition, and pooled cells were analyzed. Eomes was markedly upregulated in CD8 SP thymocytes (B), and PLZF was not expressed in CD4 SP thymocytes (C). Representative data from two independent experiments are shown. Numbers in quadrants indicate the percentages of cells in each. *p < 0.05, **p < 0.01, ***p < 0.001. n.s., not significant.