. Author manuscript; available in PMC: 2011 Oct 13.

Published in final edited form as: J Immunol. 2011 Mar 28;186(9):5304–5312. doi: 10.4049/jimmunol.1000224

Table II.

Frequency of high-affinity and low-affinity DNA-reactive B cells in R4A Tg mice treated with E2 with or without DNase

	Placebo (%)	E2 (%)	E2 + DNase (%)	E2 + HI DNase (%)
Transitional	6/50 (12)	12/52 (23)^*	8/63 (12.6)^ns	13/47 (29.7)^*
Mature
High affinity	5/60 (8.3)	18/62 (27.7)^*	8/72 (11.1)^ns	13/57 (22.8)^*
Low affinity	11/60 (18)	5/62 (8)^*	18/72 (25)	4/57 (7)^*

A significant increase in high-affinity anti-DNA B cells in R4A Tg mice treated with E2 was observed and was abrogated by treatment with DNase, but not HI DNase. Fisher’s exact test was used to analyze significance between the various treatment groups compared with the P-treated group.

p < 0.05.

ns, Not significant.