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Effect of IDO on metalloproteinase expression. (A) IDO sense and antisense-transfected P19 cells were aggregated in 1% DMSO for 30 hours before total RNA was isolated and metalloproteinase gene expression assayed by RT-PCR. Lanes: 1-antisense clone E6, 2-antisense clone D3, 3-antisense clone C2, 4-sense. (B) Collagenase gene expression in IDO-expressing RAW transfectants. V: vector-only, 6,8, 11, 22: IDO expressing RAW clones.
