Figure 2.

Molecular mass scale for the gel shown in Figure 1. The numbers refer to molecular masses (in kD). Masses used for calibration are given in red. BN gel dimension (horizontal) is as follows: I+III₂ supercomplex (1,500 kD), complex I (1,000 kD), dimeric complex III (500 kD), complex II (160 kD), and aconitate hydratase (110 kD). SDS gel dimension (vertical) is as follows: 75-kD subunit of complex I (spot 171), α-MPP (52 kD; spot 47), prohibitin (30 kD; spot 175), FeS subunit of complex III (23 kD; spot 53), QCR7 subunit of complex III (14 kD; spot 54), and the QCR8 subunit of complex III (8 kD; spot 56). Masses between those used for calibration were calculated with Eureqa software (version 0.83 β; www.eureqa.com).