| 1 |
Spot number |
Spot number on the 2D BN/SDS gel (Figure 3) |
| 2 |
Mascot score |
Probability score for the protein identification (www.matrixscience.com) |
| 3 |
No. of peptides |
No. of unique peptides matching to a protein hit |
| 4 |
Coverage |
Sequence coverage of a protein by the identified peptides |
| 5 |
Calculated molecular mass |
Calculated molecular mass of the protein (note that in many cases, presequences are cleaved off from nucleus-encoded mitochondrial proteins after their transfer into mitochondria is completed; as a consequence, the apparent molecular mass is 1–8 kD smaller than predicted) |
| 6 |
Apparent molecular mass, second dimension |
Apparent molecular mass as determined on the second gel dimension (for mass determination, see Fig. 2) |
| 7 |
Apparent molecular mass, first dimension |
Apparent molecular mass as determined on the first gel dimension (for mass determination, see Fig. 2) |
| 8 |
Accession number, Arabidopsis Genome Initiative |
Accession numbers according to the TAIR database (http://www.arabidopsis.org/); numbers are linked to protein entries of the TAIR database |
| 9 |
Name of protein |
|
| 10 |
Protein complex |
Assigned mitochondrial protein complex (in many cases according to precise location on the same vertical line on the 2D BN/SDS gel) |
| 11 |
Physiological category |
Seven categories were defined: (a) oxidative phosphorylation; (b) pyruvate metabolism and TCA cycle; (c) transport; (d) protein folding and processing; (e) processing of nucleic acids; (f) other metabolic pathways; and (g) uncharacterized |
| 12 |
Subcellular localization |
Subcellular localization according to the SUBA II database (http://suba.plantenergy.uwa.edu.au/) |
| 13 |
Database |
Database used for protein identification (T = TAIR [http://www.arabidopsis.org/], database release 10) |
| 14 |
Coordinate on the x axis |
Generated by Delta 2D |
| 15 |
Coordinate on the y axis |
Generated by Delta 2D |
| 16 |
Spot number |
Copy of column 1 for better readability of the table |
