Table II. The protein complex proteome of Arabidopsis mitochondria.
Protein Complex | Apparent Molecular Massa | Composition | No. of Different Subunits Included in the Mapb (+No. of Additional Subunits Not Found in This Study) | Additional Isoformsc | |
kD | |||||
I+III2 supercomplex | 1,500 | Heterooligomer | All subunits of complex I and III | – | |
Complex I | 1,000 | Heterooligomer | 44 + 3d | (+4) | 8 |
Prohibitin | 1,000 | Homooligomer | 1 | 6 | |
HSP60 complex | 783 | Homo-14-mer | 1 | 5 | |
Membrane arm, complex I | 660 | Heterooligomer | Approximately 35 subunits of complex I | – | |
Complex V | 660 | heterooligomer | 14 | (+1) | 2 |
Complex III | 500 | Heterooligomer | 10 | 6 | |
PPR2 complex | 369 | Homooligomer (?) | 1 + X | 1 | |
NAD malic enzyme | 369 | Heterohexamer | 2 | 0 | |
F1 part of complex V | 300 | Heterooligomer | Five subunits of complex V | – | |
TOM complex | 260 | Heterooligomer | 4 | (+2) | 6 |
F0 part of complex V | 260 | Heterooligomer | Seven subunits of complex V | – | |
Clp protease complex | 260 | Homo-14-mer | 1 | 0 | |
Oxoglutarate DH-E1 | 210 | Homodimer | 1 | 1 | |
PPR3 complex | 209 | Homooligomer (?) | 1 + X | 0 | |
Lon protease | 209 | Homodimer | 1 | 3 | |
Glu dehydrogenase | 209 | Homohexamer | 1 | 2 | |
Complex IV | 207 | Heterooligomer | 6 | (+8) | 8 |
Fumarase | 200 | Homotetramer | 1 | 1 | |
Complex II | 160 | Heterooligomer | 7 | (+1) | 5 |
PPR1 complex | 160 | Homooligomer (?) | 1 + X | 0 | |
Isocitrate DH | 160 | Homotetramer | 1 | 3 | |
Malate DH | 160 | Homotetramer | 1 | 2 | |
Alternative ND (NDA + NDB) | 160 | Heterotrimer | 2 | 4 | |
PPR5 complex | 156 | Homooligomer (?) | 1 + X | 1 | |
Metaxin/SAM complex (?) | 150 | Heterooligomer | 1 | 0 | |
Arginase | 150 | Homotrimer | 1 | 1 | |
Pyruvate DH-E1 | 138 | Heterotetramer | 2 | 1 | |
Isovaleryl-CoA dehydrogenase | 132 | Homotetramer (?) | 1 | 0 | |
TIM23 complex | 115 | Heterooligomer | 2 | (+4) | 2 |
Citrate synthase | 110 | Homodimer | 1 | 1 | |
HSP10 complex | 108 | Homoheptamer | 1 | 1 | |
Voltage-dependent anion channel complexes | 90/180 | Homotri/hexamers | 1 | 4 | |
ADP/ATP translocase | 60/120 | Homodi/tetramers | 1 | 2 | |
TIM9-10 complex | 86 | Heterohexamer | 2 | 0 | |
Σe (35 complexes) | 86–1,500 | 118 | (+20) | 76 |
Apparent molecular mass as determined in this study.
Without isoforms.
Based on the genome sequence. Note that not all isoforms were detected in the frame of this project.
Three new complex I subunits were discovered in the frame of this project.
Subunits detected in a holocomplex and in subcomplexes only were counted once.