Table I.

Genes identified in the reverse genetics screen that, when mutated, affect pollen and anther morphology

Gene	Primary-Screen Mutant Phenotype	Exine Mutant Phenotypea	Annotation/Function	Putative Pathway	Expression Patternb	Insertion Lines Tested
At1g33430	Glossy/sticky pollen	upex (allelic to upex1 and upex2)c	Glycosyl transferase		Buds (stages 9–12), not in ap3 and ag	SALK_091466
At1g65060	Glossy/sticky pollen	mlp5	4-Coumarate-CoA ligase (4CL3)	Phenolics	Buds (stages 9–10), petals	SALK_017894, WiscDsLox503E07
At1g69500	Glossy/sticky pollen	zebra (allelic to lines 28-3-1, 37-2-3, 119-1-1, 131-3-1, 155-4-1, 174-154-2)c	CYP704B1, fatty acid ω-hydroxylase	Fatty acid	Buds (stages 9–10), not in ap3 and ag	SAIL_1149_B03
At2g19070	Glossy/sticky pollen	mlp6	Spermidine hydroxycinnamoyl transferase	Phenolics (spermidine conjugates)	Buds (stages 9–10), not in ap3 and ag	SALK_055511
At3g28780	Glossy/sticky pollen	mlp7	Gly-rich protein		Stamen	GT_5_86366
At4g14080	Glossy/sticky pollen	mlp (allelic to mlp4)c	β-1,3-Glucanase/glycosyl hydrolase (A6/MEE48)	Callose degradation	Buds (stages 9–10), not in ap3 and ag	WiscDSLox285F04
At5g13930	White pollen	mlp8	Chalcone synthase TT4	Phenolics (flavonoids)	Multiple tissues and stages	SALK_020583

^aPhenotypic categories developed in the secondary screen with confocal microscopy. For descriptions of the different categories, see “Results.”

^bBased on expression data from the microarray (e.g. GENEVESTIGATOR [Zimmerman et al., 2004] and/or Massively Parallel Signature Sequencing [Meyers et al., 2004]) databases.

^cIndicates additional alleles that were found during the forward genetic screen.