Figure 3.

Characterization of the hcf243 mutant. A, RT-PCR analysis of HCF243 gene expression. RT-PCR was performed with actin-specific primers and the specific primers for At3g15095, At3g15090, and At3g15110. B, Northern-blot analysis of HCF243 gene expression in wild-type (WT) and hcf243 plants. Thirty micrograms of total RNA from wild-type and hcf243 plants was size fractionated by agarose gel electrophoresis, transferred to a nylon membrane, and probed with ³²P-labeled HCF243 cDNA probe. rRNAs were detected by ethidium bromide staining. C, Immunodetection of HCF243 with the specific HCF243 antibody.