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. 2011 Aug 23;157(2):608–619. doi: 10.1104/pp.111.183301

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© 2011 American Society of Plant Biologists. All rights reserved.

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Figure 7. — BiFC detection of HCF243 and D1 protein interactions and their subcellular localization. GFP-tagged HCF243, nYFP-tagged HCF243, and cYFP-tagged D1 constructs were transiently expressed in leaf epidermal cells of N. benthamiana plants, and their interaction and subcellular locations were examined with a laser scanning confocal microscope. Green fluorescence indicates GFP, red fluorescence shows chloroplast autofluorescence, and orange/yellow fluorescence shows images with the two types of fluorescence merged. A, N. benthamiana leaf epidermal cells expressing YFP-tagged HCF243 constructs. B, Interaction between nYFP-tagged HCF243 and cYFP-tagged D1 in chloroplast of N. benthamiana leaf epidermal cells. C, nYFP and cYFP constructs were coexpressed in N. benthamiana leaf epidermal cells as a negative control, showing no signal. D and E, nYFP-tagged HCF243 (D) and cYFP-tagged D1 (E) constructs were expressed alone in N. benthamiana leaf epidermal cells as a negative control, showing no signal. Bars = 10 μm.