Table II. Disc diffusion assay for antimicrobial activities.
According to the experimental scheme shown in Figure 1A, samples were obtained at different time points (h) from tobacco leaves preinfiltrated with A. tumefaciens with (ipt) or without (0) the 4xJERE:ipt construct followed by infiltration of PstT (Pst) or a mock infiltration (0). Sample = sampling time-first infiltration-second infiltration. d.o., Disc overgrown. Values are means of two independent experiments.
| Diameter of Inhibition Zone |
||||||||||
| Sample No. According to Figure 1A | Sample | Gram-Positive Bacteria |
Gram-Negative Bacteria | Fungi |
||||||
| B. subtilis | S. aureus | E. coli | P. aeruginosa | Psp | Pst | C. maltosa | B. cinerea | S. sclerotiorum | ||
| mm | ||||||||||
| 1 | 0-0-0 | 1 | 2 | 0 | 1 | 1 | 1 | 1 | 0 | 0 |
| 2 | 24-ipt-0 | 2 | 2 | 1 | 2 | 2 | 2 | 1 | d.o. | 0 |
| 3 | 24-0-0 | 1 | 1 | 0 | 2 | 1 | 2 | 1 | 0 | 0 |
| 8 | 48-ipt-Pst | 3 | 3 | 7 | 4 | 8 | 9 | 3 | d.o. | 0 |
| 9 | 48-0-Pst | 2 | 2 | 1 | 2 | 3 | 3 | 2 | 0 | 0 |