Table III. Scopoletin and capsidiol production is stimulated by CK.
According to the experimental scheme shown in Figure 1A, samples were obtained at different time points (h) from tobacco leaves preinfiltrated with A. tumefaciens with (ipt) or without (0) the 4xJERE:ipt construct followed by infiltration of PstT (Pst) or a mock infiltration (0). Sample = sampling time-first infiltration-second infiltration. N.D., Not determined. Mean values of two technical replicates ± sd from one biological sample are shown.
Sample No. According to Figure 1A | Sample | Scopoletin | Ratio ipt:0 | Capsidiol | Ratio ipt:0 |
nmol g−1 fresh wt | nmol g−1 fresh wt | ||||
1 | 0-0-0 | 3.1 ± 1.21 | 4.3 ± 0.98 | ||
2 | 24-ipt-0 | 7.4 ± 2.94 | 1.10 | N.D. | N.D. |
3 | 24-0-0 | 6.7 ± 3.64 | N.D. | ||
4 | 30-ipt-Pst | 11.3 ± 4.33 | 1.20 | N.D. | N.D. |
5 | 30-0-Pst | 9.4 ± 4.85 | N.D. | ||
6 | 36-ipt-Pst | 24.3 ± 1.56 | 1.90 | N.D. | N.D. |
7 | 36-0-Pst | 12.8 ± 1.56 | N.D. | ||
8 | 48-ipt-Pst | 78.8 ± 10.28 | 6.40 | 74.5 ± 6.04 | 4.63 |
9 | 48-0-Pst | 12.3 ± 1.85 | 16.1 ± 2.66 |