Figure 4.

Immunofluorescence microscopy of protein bodies in the wild type and the glup4 mutant, EM956. A and C, The wild type. B, D, and E, glup4 (EM956). Secondary antibodies labeled with rhodamine (magenta) and fluorescein isothiocyanate (FITC; green) were used to visualize the reaction of prolamin and glutelin antibodies, respectively, in A and B. Secondary antibodies labeled with rhodamine and FITC were used to detect antigen recognized by anti-α-globulin and anti-glutelin, respectively, in C, D, and E. Arrowheads indicate the presence of the novel large distended structures containing proglutelin and α-globulin. In many instances, the large distended structures are clustered in neighboring cells (B). Glutelins are present in overlapping layers (B and D). Also note that the distribution of proglutelins and globulin within the large distended structures is spatially distinct (D). Bars = 10 μm.