Figure 7.

Identification of the major products of TPS3 enzymatic activity in vitro as camphene and tricyclene, and correlation with the presence of these compounds and of transcripts of TPS3 in leaf and stem tissues of tomato. A, TPS in vitro activity with GPP as the substrate (top panel) and GC analysis of monoterpenes extracted from leaves and stems by SPME (bottom two panels). B, RT-PCR analysis of TPS3 transcripts in leaves and stems. The reaction products (peaks) were identified by comparison of their GC-MS profiles with authentic standards and NIST libraries: (1) tricyclene, (2) α-pinene, (3) camphene, (4) sabinene, (5) β-pinene, (6) β-myrcene, (7) limonene, (8) unidentified. TIC, Total ion chromatograph.