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. 2011 Aug 18;157(2):917–936. doi: 10.1104/pp.111.176818

Table I.

Proteins that showed significant changes in ZmASR1-OE leaves compared with wild-type leaves

Spot IDa Trend in ZmASR1-OE Ratio of ZmASR1-OE to the Wild Typeb ANOVA Tablec Maize Gene Modeld Annotatione Class
Control Deficit ZmASR1-OE Deficit Interaction
s0732 Down 0.99 0.34 NR NR 0.007 NI ND ND
s1021 Up 1.30 2.22 0.020 0.752 0.071 GRMZM2G019121 Class II aspartyl-tRNA synthetase (AspRS) Amino acid activation
s1202 Up 2.94 1.74 0.020 0.420 0.561 GRMZM2G008247 β-d-Glucosidase (GLU) Carbohydrate metabolism
GRMZM2G034152 Flavin-containing amine oxidase
s1388 Down 0.50 0.55 0.006 0.304 0.578 GRMZM2G127393 Trigger factor-like protein Protein folding
s1406 Down 0.13 0.73 NR NR 0.027 GRMZM2G123204 Adenylosuccinate synthetase (AdSS) Purine biosynthesis
GRMZM2G020446 Diaminopimelate decarboxylase
s1422 Down 0.42 0.98 NR NR 0.031 GRMZM2G123204 Adenylosuccinate synthetase (AdSS) Purine biosynthesis
s1442 Down 0.26 0.56 0.015 0.491 0.118 NI ND ND
s1444 Down 0.66 0.74 0.005 0.289 0.686 GRMZM2G449496 Plastid transcriptionally active 16 (PTAC16) RNA regulation
s1612 Down 0.37 0.86 NR NR 0.001 GRMZM2G104613 3-Isopropylmalate dehydrogenase 2 (IPMDH2) Leu biosynthesis
GRMZM2G803490 3-Isopropylmalate dehydrogenase 1 (IPMDH1)
s1641 Down 0.48 0.88 NR NR 0.026 GRMZM2G803490 3-Isopropylmalate dehydrogenase 1 (IPMDH1) Leu biosynthesis
s1886 Down 0.68 0.65 0.019 0.248 0.953 GRMZM2G097226 Pyruvate dehydrogenase subunit E1β (PDH-E1β) Glycolysis and tricarboxylic acid cycle
s1904 Down 0.70 0.63 0.007 0.017 0.859 AC147602.5_FGP004 Sedoheptulose-1,7-bisphosphatase (SBPase) Calvin cycle
s1913 Down 1.14 0.68 NR NR 0.001 GRMZM2G102838 PSII stability/assembly factor (HCF136) Protein assembly
s2020 Down 0.99 0.50 NR NR 0.010 AC210204.3_FGP002 C2 domain-containing protein Stress
s2141 Down 0.30 0.68 0.005 0.035 0.150 NI ND ND
s2210 Down 0.71 0.38 0.019 0.215 0.258 GRMZM2G018375 Thiazole biosynthetic enzyme 1-1 (THI1) Thiamine biosynthesis
s2275 Up 1.82 1.23 0.050 0.013 0.055 GRMZM2G064163 Spermidine synthase 1 Polyamine biosynthesis
s2316 Up 1.56 1.29 0.011 0.028 0.133 GRMZM2G078566 Glucan endo-1,3-β-glucosidase 5 Carbohydrate metabolism
s2322 Up 1.75 1.34 0.005 0.436 0.108 NI ND ND
s2460 Down 0.82 0.63 0.035 0.023 0.112 NI ND ND
s2528 Up 1.54 1.21 0.020 0.778 0.244 GRMZM2G068244 NAD-dependent epimerase/dehydratase Coenzyme binding
s3353 Up 1.85 1.32 0.020 0.306 0.170 NI ND ND
a

Identification number of the corresponding protein spot on the two-dimensional reference map.

b

The expression values are reported relative to the wild-type samples in the same culture condition (n = 2).

c

Proteins were categorized as ZmASR1-OE (boldface text), deficit (underlined text), and interaction (italicized text) as follows: ZmASR1-OE, proteins that showed significant (P < 0.05) changes in ZmASR1-OE leaves compared with wild-type leaves when the additive model could be retained; deficit, proteins that showed significant (P < 0.05) changes under water deficit conditions compared with well-watered conditions when the additive model could be retained; interaction, proteins that showed significant (P < 0.05) changes in ZmASR1-OE leaves compared with wild-type leaves by the Bonferroni method when the additive model could not be retained. Experimental details are described in “Materials and Methods.” NR, Not relevant.

d

Maize genome release 5a.59 of November 2010 (http://www.maizesequence.org). NI, Not identified by LC-MS/MS.

e

Manually improved annotation from SwissProt, GenBank, Trembl, and InterPro databases. Annotations in italicized text were eliminated based on the absence of correspondence between the theoretical Mr and the observed Mr and/or the protein abundance index (see Supplemental Table S6). ND, Not determined.