Table I.
Spot IDa | Trend in ZmASR1-OE | Ratio of ZmASR1-OE to the Wild Typeb | ANOVA Tablec | Maize Gene Modeld | Annotatione | Class | |||
Control | Deficit | ZmASR1-OE | Deficit | Interaction | |||||
s0732 | Down | 0.99 | 0.34 | NR | NR | 0.007 | NI | ND | ND |
s1021 | Up | 1.30 | 2.22 | 0.020 | 0.752 | 0.071 | GRMZM2G019121 | Class II aspartyl-tRNA synthetase (AspRS) | Amino acid activation |
s1202 | Up | 2.94 | 1.74 | 0.020 | 0.420 | 0.561 | GRMZM2G008247 | β-d-Glucosidase (GLU) | Carbohydrate metabolism |
GRMZM2G034152 | Flavin-containing amine oxidase | ||||||||
s1388 | Down | 0.50 | 0.55 | 0.006 | 0.304 | 0.578 | GRMZM2G127393 | Trigger factor-like protein | Protein folding |
s1406 | Down | 0.13 | 0.73 | NR | NR | 0.027 | GRMZM2G123204 | Adenylosuccinate synthetase (AdSS) | Purine biosynthesis |
GRMZM2G020446 | Diaminopimelate decarboxylase | ||||||||
s1422 | Down | 0.42 | 0.98 | NR | NR | 0.031 | GRMZM2G123204 | Adenylosuccinate synthetase (AdSS) | Purine biosynthesis |
s1442 | Down | 0.26 | 0.56 | 0.015 | 0.491 | 0.118 | NI | ND | ND |
s1444 | Down | 0.66 | 0.74 | 0.005 | 0.289 | 0.686 | GRMZM2G449496 | Plastid transcriptionally active 16 (PTAC16) | RNA regulation |
s1612 | Down | 0.37 | 0.86 | NR | NR | 0.001 | GRMZM2G104613 | 3-Isopropylmalate dehydrogenase 2 (IPMDH2) | Leu biosynthesis |
GRMZM2G803490 | 3-Isopropylmalate dehydrogenase 1 (IPMDH1) | ||||||||
s1641 | Down | 0.48 | 0.88 | NR | NR | 0.026 | GRMZM2G803490 | 3-Isopropylmalate dehydrogenase 1 (IPMDH1) | Leu biosynthesis |
s1886 | Down | 0.68 | 0.65 | 0.019 | 0.248 | 0.953 | GRMZM2G097226 | Pyruvate dehydrogenase subunit E1β (PDH-E1β) | Glycolysis and tricarboxylic acid cycle |
s1904 | Down | 0.70 | 0.63 | 0.007 | 0.017 | 0.859 | AC147602.5_FGP004 | Sedoheptulose-1,7-bisphosphatase (SBPase) | Calvin cycle |
s1913 | Down | 1.14 | 0.68 | NR | NR | 0.001 | GRMZM2G102838 | PSII stability/assembly factor (HCF136) | Protein assembly |
s2020 | Down | 0.99 | 0.50 | NR | NR | 0.010 | AC210204.3_FGP002 | C2 domain-containing protein | Stress |
s2141 | Down | 0.30 | 0.68 | 0.005 | 0.035 | 0.150 | NI | ND | ND |
s2210 | Down | 0.71 | 0.38 | 0.019 | 0.215 | 0.258 | GRMZM2G018375 | Thiazole biosynthetic enzyme 1-1 (THI1) | Thiamine biosynthesis |
s2275 | Up | 1.82 | 1.23 | 0.050 | 0.013 | 0.055 | GRMZM2G064163 | Spermidine synthase 1 | Polyamine biosynthesis |
s2316 | Up | 1.56 | 1.29 | 0.011 | 0.028 | 0.133 | GRMZM2G078566 | Glucan endo-1,3-β-glucosidase 5 | Carbohydrate metabolism |
s2322 | Up | 1.75 | 1.34 | 0.005 | 0.436 | 0.108 | NI | ND | ND |
s2460 | Down | 0.82 | 0.63 | 0.035 | 0.023 | 0.112 | NI | ND | ND |
s2528 | Up | 1.54 | 1.21 | 0.020 | 0.778 | 0.244 | GRMZM2G068244 | NAD-dependent epimerase/dehydratase | Coenzyme binding |
s3353 | Up | 1.85 | 1.32 | 0.020 | 0.306 | 0.170 | NI | ND | ND |
Identification number of the corresponding protein spot on the two-dimensional reference map.
The expression values are reported relative to the wild-type samples in the same culture condition (n = 2).
Proteins were categorized as ZmASR1-OE (boldface text), deficit (underlined text), and interaction (italicized text) as follows: ZmASR1-OE, proteins that showed significant (P < 0.05) changes in ZmASR1-OE leaves compared with wild-type leaves when the additive model could be retained; deficit, proteins that showed significant (P < 0.05) changes under water deficit conditions compared with well-watered conditions when the additive model could be retained; interaction, proteins that showed significant (P < 0.05) changes in ZmASR1-OE leaves compared with wild-type leaves by the Bonferroni method when the additive model could not be retained. Experimental details are described in “Materials and Methods.” NR, Not relevant.
Maize genome release 5a.59 of November 2010 (http://www.maizesequence.org). NI, Not identified by LC-MS/MS.
Manually improved annotation from SwissProt, GenBank, Trembl, and InterPro databases. Annotations in italicized text were eliminated based on the absence of correspondence between the theoretical Mr and the observed Mr and/or the protein abundance index (see Supplemental Table S6). ND, Not determined.