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. 2011 Aug 9;157(2):683–691. doi: 10.1104/pp.111.180083

Figure 6.

Figure 6.

Regulation in trans of mRNAs unrelated to TAAR mRNAs by siTAARs. A, Sites in the coding regions of At5g24650 and LPAT5 (At3g18850) for cleavage guided by the siTAARs AFB2 3′D1(+), AFB3 3′D1(+), AFB3 3′D6(+), and AFB2 3′D6(+). Perfect (I) and wooble (o) base pairing are indicated. The cleavage sites (vertical arrows) and number of sequence reads obtained in the PARE dataset by German et al. (2008) are indicated. The ΔG values for target-sRNA pairs were calculated using the DINAMelt server. B, Real-time reverse transcription-qPCR of At5g24650 (white bars) and LPAT5 (black bars) mRNAs in mutants affected in siTAAR production. mRNA contents are expressed as fold wild type relative to the ACTIN2 standard. Error bars, sem for two or three measurements of total RNA obtained from pools of at least 20 plants; *, significantly different from wild type (P < 0.05, t test of means). The primers used span the siTAAR complementary sites.