Endothelial cell (HUVEC) (A) viability and (B) density as a function of culture duration and extent of albumin glycation. Viability and density were calculated from 3–4 images from 4–5 independent experiments. Each bar represents the mean + standard error of the mean of the pooled data from each experiment. +, significantly different from no added albumin (paired by culture duration, two-way ANOVA, p < .05); *, significantly different from day 3 (paired by treatment, two-way ANOVA, p < .05); #, significantly different from nonglycated albumin (paired by culture duration, two-way ANOVA, p < .05).