Figure 1.

Intracellular ROS generation in response to HSV-1 infection of primary microglia. Purified murine microglial cell cultures were either left uninfected (Control) or infected with HSV-1 (MOI = 2.5) for 3 or 24 h prior to loading with H₂DCFDA (20 μM, 45 min) for visualization using fluorescence microscopy. Data shown are representative of five individual experiments using microglial cells obtained from different animals.