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Anatomical localization of Lcn2 and 24p3R RNA in the brain following intraperitoneal injection of LPS. Brain was removed from mice at different times following LPS injection, fixed in ice-cold 4% PBS-buffered paraformaldehyde and paraffin-embedded sagittal sections (10 μm) subjected to ISH as described in the Materials and Methods. Hybridization was performed with either 24p3R (A&B) and Lcn2 (D&E) anti-sense or 24pR3 (C) and Lcn2 (F) sense cRNA probes.
