Figure 4.

Cellular localization of Lcn2 and 24p3R RNA in the brain following intraperitoneal injection of LPS. Paraffin-embedded sagittal sections (10 μm) were subjected to ISH followed by immunohistochemistry as described in the Materials and Methods. A. Brain section from vehicle-injected control mouse hybridized with Lcn2 anti-sense cRNA probe and immunostained for NeuN. B-D. Brain sections from dual LPS-treated mice hybridized with Lcn2 anti-sense cRNA probe and immunostained for NeuN (B) and lectin (C&D). E-F. Brain sections from vehicle-injected control mice hybridised with 24p3R anti-sense cRNA probe and immunostained for NeuN (E) or lectin (F).