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. 2011 Sep 29;8:125. doi: 10.1186/1742-2094-8-125

Figure 2.

Figure 2

Curcumin induces genes related to adhesion and anti-inflammatory response. Real-time qRT-PCR validation of transcripts in BV-2 microglia stimulated with 20 μM curcumin, 100 ng/ml LPS, or 20 μM curcumin + 100 ng/ml LPS for 6 hours. Relative mRNA levels were quantified for Netrin G1 (Ntng1), Platelet endothelial call adhesion molecule 1 (Pecam1), Delta-like 1 (Dll1), Plasma cell induced endoplasmic reticulum protein 1 (Perp1), Peroxisome proliferator activated receptor α (PPARα), and Interleukin 4 (Il4). Expression was normalized to the control gene Atp5b and mRNA levels (+/- SD) are graphed relative to control cells. Results are calculated from three independent experiments performed in triplicate measurements. * p ≤ 0.05, *** p ≤ 0.001 for curcumin vs. control, Mann-Whitney Rank Sum test. n.e., not expressed.