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. 2011 Oct 13;6(10):e25761. doi: 10.1371/journal.pone.0025761

Figure 2. Expression and differentiation analysis.

Figure 2

A: Expression of the virally-transduced reprogramming factors cDNA. Y-axis represent absolute number of copies of cDNA per ng of RNA determined using a standard curved based on a purified PCR product. Expression of the transduced cDNA was mostly silent except for FL iPS C (see text). B: Global expression patterns: Scatter-plot illustrating an Affymetrix micro-array analysis of iPS as compared to H1 and to parental somatic cells. Micro-array results were normalized by RMA. Average of two biological replicates is plotted. Left scatter-plot illustrates expression of H1 and H9 cells, middle scatter-plot, expression of iPSA versus p51R parental cells and right scatter plot expression of iPSA versus H1. iPS are undistinguishable from ES cells in this assay. All iPS were at least at passage 20 when the RNA were extracted. C: Embryoid bodies. Phase contrast micrographs illustrating 5 days EB formed from iPS. D: Spontaneous differentiation: Immuno-fluorescence micrographs illustrating iPS differentiation into the three germ layers. Two days EB were transferred to chamber slides containing ES medium without bFGF. After 7 days, the differentiated cells were stained with FITC labeled antibodies against β-III-tubulin (ectoderm), α-smooth muscle actin (mesoderm) and α-feto-protein (endoderm) and counter-stained with DAPI. Results from 2 different iPS are shown. (Bar = 100μm). E: Micrographs illustrating H and E stained slide of teratomas generated from an iPS. iPS can differentiate into teratomas containing the three germ layers (see also Figure S5).