Table 2.
Applied antibodies and retrieval systems
| Primary antibodies | Cat. # (clone) | Manufacturer | Dilution | Retrieval system |
|---|---|---|---|---|
| CK7 | M7018 (OV-TL 12/30) | Dako | 1:50 | Retrieval solution, High pH* |
| CK19 | M0888 (RCK108) | Dako | 1:50 | Retrieval solution, High pH* |
| HepPar1 | M7158 (OCH1E5) | Dako | 1:35 | Retrieval solution, High pH* |
| EpCAM | M0804 (BER-EP4) | Dako | 1:200 | Retrieval solution* |
| Laminin | Z0097 | Dako | 1:500 | Proteinase K, ready to use+ |
| SOX9 | HPA001758 | Sigma | 1:25 | Retrieval solution* |
| AQP1 | AB3065 | Chemicon | 1:1200 | |
| Nestin | MAB5326 (10C2) | Chemicon | 1:300 |
*Sections were boiled in Dako retrieval solution (S3307/S1699) for 10 minutes in a microwave oven and let to cool to room temperature for 20 minutes
+Sections incubated with Dako proteinase K (S3020) for 2½ minutes at room temperature.
Utilized secondary antibodies from Dako were anti-mouse (K4000) or anti-rabbit (K4002).