Table 1. 454 GS FLX and Illumina GAIIx sequencing statistics of three single unfed adult I. ricinus ticks and number of sequence reads matching to reference genomes.
| Tick sample | Type of nucleic acids | Sequencing statistics | Reference genomes | No. of reads matching to ref. genomes |
| Ir 1-4 | Genomic DNA | 454 GS FLX (2 quad) | B. burgdorferi s.s. | 1a |
| Total no. reads: 127,974 | B. azelii | 0 | ||
| Average read length: 159.8 bp | B. garinii | 0 | ||
| Ir 1-4 | cDNA | 454 GS FLX (1 quad) | B. burgdorferi s.s. | 0 |
| Total no. reads: 60,186 | B. azelii | 1a | ||
| Average read length: 227.4 bp | B. garinii | 0 | ||
| Ir 13-4 | Genomic DNA | Illumina GAIIx (1 lane) | B. burgdorferi B31 b | 393c |
| Total no. reads: 56,657,258 | B. azelii PKo b | 652c | ||
| Read length: 76 bpe | B. garinii PBi b | 4,652d | ||
| B. garinii PBr b | 4,726d | |||
| Ir 20-8 | Genomic DNA | Illumina GAIIx (1 lane) | B. burgdorferi B31 b | 112c |
| Total no. reads: 46,555,380 | B. azelii PKo b | 2,313d | ||
| Read length: 76 bpe | B. garinii PBi b | 114c | ||
| B. garinii PBr b | NA |
a
Reads identified by the comparison against the NCBI non-redundant protein database using BLASTX and assigned to taxa by MEGAN. Bit score cutoff ≥40.
b
GenBank accession number of the four chromosome genomes used as reference genomes: B. burgdorferi B31 NC_001318, B. afzelii PKo NC_008277, B. garinii PBi NC_006156 and B. garinii PBr WGS ABJV00000000.
c
Number of reads mapped to the reference genomes by BWA (mapping quality score ≥15).
d
Number of reads mapped to the reference genomes by inGAP.
e
Paired-end reads.