Table 1. The top seventy differentially expressed genes during a temperature shift from 42°C to 50°C at 15 min and 30 min in S. thermophilus.

Gene ID	COGs	Gene	Fold Change at 50°C		Description
			15 min	30 min
stu0120	O	DnaK	17.3	19.8	molecular chaperone
stu0119	O	GrpE	14.5	17.6	heat shock protein
stu0121	O	DnaJ	10.5	11.6	chaperone protein
stu0203	O	GroES	8.1	10.5	co-chaperonin
stu0204	O	GroEL	5.4	5.3	chaperonin
stu1614	O	ClpL	3.2	2.7	ATP-dependent Clp protease
stu0356	O	clpP	1.9	3.2	ATP-dependent Clp protease
stu0705	V	hsdR1	4.5	4.6	type I restriction-modification system
stu0317	TK	CovR	1.8	4.2	response regulator
stu1802	R	-	7.6	4.6	hypothetical protein
stu1875	R	-	2.8	4.3	hypothetical protein
stu1666	R	-	−2.7	−3.3	putative ABC transporter ATP binding protein
stu1665	R	-	−3.3	−2.1	putative ABC transporter permease protein
stu1615	R	-	−3.5	−3.7	hypothetical protein
stu1005	P	pstB2	5.3	5.6	phosphate transport system ATP-binding protein
stu1002	P	pstC1	4.5	3.2	phosphate transport system permease protein
stu1003	P	pstC2	3.8	2	phosphate transport system permease protein
stu0607	P	feoA	2.7	3.5	ferrous iron uptake transporter protein A
stu1025	P	fatB	2.4	3.1	iron complex transport substrate-binding protein
stu0724	P	Fur	−3	−3.4	transcriptional regulator
stu0569	M	-	4.5	4.3	glycosyl transferase family protein
stu0762	M	dltB	−2.8	−3.8	integral membrane protein
stu1465	L	radC	3.1	2.5	DNA repair protein
stu0118	K	hrcA	6.8	4.6	heat-inducible transcription repressor
stu0065	K	MarR	3.8	5.2	transcriptional regulator
stu0432	K	MarR	1.8	3.1	transcriptional regulator
stu1600	K	MerR	−1.5	−4.3	transcriptional regulator
stu0931	K	TetR	−1.6	3.3	transcriptional regulator
stu1868	K	rpoB	−3.8	−3.4	DNA-directed RNA polymerase
stu0076	K	CtsR	−4.3	−4.8	transcriptional regulator
stu0419	J	-	5.4	4.5	acetyltransferase
stu0094	J	rpsI	4.2	2.3	30S ribosomal protein S9
stu0074	J	tsf	3.6	3.1	elongation factor Ts
stu0451	J	metG	3.2	4.3	methionyl-tRNA synthetase
stu1808	J	rpmH	3.1	2.8	50S ribosomal protein L34
stu1932	J	rplW	−2.6	3.6	50S ribosomal protein L23
stu1926	J	rpmC	−3.3	−3.6	50S ribosomal protein L29
stu0151	J	def	−3.4	−3.5	peptide deformylase
stu1814	J	gltX	−3.6	−3.1	glutamyl-tRNA synthetase
stu0154	J	rpsO	−5.3	−5	30S ribosomal protein S15
stu1196	G	pyk	−1.8	−4.2	pyruvate kinase
stu0807	F	cdd	2.4	3.8	cytidine deaminase
stu1316	E	sdaB	4.9	2.5	L-serine dehydratase beta subunit
stu1878	E	thrC	3.4	3	threonine synthase
stu0363	E	livF	1.8	3.8	branched-chain amino acid transport system ATP-binding protein
stu0159	E	-	−3.6	−3.1	polar amino acid transport substrate-binding protein
stu1461	E	nifS3	−5	−3.5	Putative cysteine desulfurase
stu0479	C	atpB	−3.2	−3.3	F0F1 ATP synthase
stu0478	C	atpE	−6.3	−4.8	F0F1 ATP synthase
stu0863	-	-	4	3.5	hypothetical protein
stu1575	-	-	4	1.6	hypothetical protein
stu1378	-	-	3.6	2	hypothetical protein
stu0087	-	-	3	1.6	hypothetical protein
stu0829	-	-	2.8	3.3	hypothetical protein
stu1722	-	-	2.8	3.4	hypothetical protein
stu0161	-	-	2.4	3.6	hypothetical protein
stu1075	-	-	1.4	3.7	hypothetical protein
stu0013	-	-	−3.2	−3.7	hypothetical protein
stu0135	-	-	−3.4	−5.4	hypothetical protein
stu1454	-	-	−3.5	−1.6	hypothetical protein
stu1533	-	-	−3.6	−3	hypothetical protein
stu1639	-	-	−3.9	−1.9	hypothetical protein
stu1047	-	-	−4.2	−3.6	hypothetical protein
stu1459	S	-	3.6	2	hypothetical protein
stu0440	S	-	2.8	4.6	hypothetical protein
stu1248	S	-	1.8	3.5	hypothetical protein
stu0423	S	-	−3.3	−2.7	hypothetical protein
stu1253	S	-	−4.1	−3.9	hypothetical protein
stu0888	S	-	−8	−5.6	hypothetical protein
stu0868	S	-	−9.3	−5.4	hypothetical protein