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EGFR upregulation can be detected as early as 24 hours in E-cad siRNA transfected cells. Cell lysate from PCI-37A cells was collected at 12, 24, and 48 hours after transfection with E-cad and control siRNAs. 50 μg protein was loaded for SDS-PAGE, and Western blot analysis was performed using EGFR antibody. The result showed EGFR protein was upregulated as early as 24 hours after siRNA was transfected.
