Table 1. Proportions of Y chromosome-positive cells in intestinal populations of female Rag2 KO mice receiving male SWR Bone Marrow.
Days after BMTx | |||||
3 | 10 | 28 | 38 | 38 | |
Small Intestine | Control | Control | GvHD-like | DSS | Control |
Stroma | 0±0 (4) | 0.65±0.07 (3) | 0.81±0.12 (10) | 0.77±0.15 (5) | 0.69 (1) |
Lymphoid Aggregates | 0±0 (4) | 0.51±0.24 (4) | 0.58±0.16 (3) | 0.72±0.08 (3) | 0.58 (1) |
Pericryptal Myofibroblasts | 0±0 (4) | 0±0 (4) *** | 0.03±0.06 (7) *** | 0.10±0.14 (2) *** | 0.20 (1) |
Figures represent the mean % scores (± SD; (n) values) for either Y chromosome and CD45 double-positive cells in stroma or lymphoid aggregates, or Y chromosome and α-SMA double-positive PCF cells in the intestines of female Rag2 KO mice receiving male SWR BMTx on day 0. Stroma includes cells between 20 µm from the crypt basal edge and the muscularis mucosae. Pericryptal myofibroblasts were those α-SMA -positive cells inside 20 µm of the crypt basement membrane. No Y-positive epithelioid cells were found which were not also CD45-positive [ie of lymphoid origin]; those cells are included within the stromal counts. Only unequivocally double-labelled cells were scored, and so these counts represent an underestimate of the true values. No corrections have been made for section thickness (5 µm). Differences from Stroma group in the same tissue:
p<0.01,
p<0.001 (Tukey-Kramer multiple comparisons post-test).