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. 2011 Oct 13;6(10):e26187. doi: 10.1371/journal.pone.0026187

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Kunze et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A1 and B1) Schematic view of shifted cell layer position and NGF/B27 (12B27pNGF-53) gradient exposure. (A2 and B2) False color image shows cell pattern dependent synapse distribution in the micropatterned cell culture after 9 DIV. Pre-synaptic units: synaptophysin (red), cell nucleus: DAPI (blue), scale bar = 100 µm. (A3 and B3) Surface plots of synapse distribution with linear fit of data demonstrate synaptic gradient response, z = 5 µm.