Figure 4. Inter-strain variation in protein levels of gH.

A) Western blot analysis of infected cell lysates demonstrates that PRV Bartha produces gH (UL22) comparable to that in virulent strains. PRV Becker displays slightly higher and/or differentially glycosylated levels of gH than the other two strains. Levels of the capsid protein VP5 (UL19) are shown for comparison and as a loading control. B) Ratio of gH vs. VP5 in each sample, using the ImageJ Gel Analyzer module. Equivalent amounts of protein were loaded in each lane. The blot was cut, with VP5 measured on the upper half and gH on the lower half to demonstrate equivalent levels of infection in each lysate. The same lysates were used for the analyses in Figure S3 (in Text S1); these are representative of three separate experiments. The conditions required to visualize the two bands of gH precluded measurement of cellular actin on the same blot. Positions of a standard marker are noted on the left.