Table 1. Protein-coding variations unique to the vaccine strain PRV Bartha, in comparison to the virulent strains PRV Becker and Kaplan.
| Gene a | Amino acid residues found in PRV Bartha, which differ from PRV Becker & Kaplan b |
| ICP27 (UL54) | G9D, T258A |
| UL52 | A824V |
| dUTPase (UL50) | S20G |
| gN (UL49.5) | L7P |
| VP22 (UL49) | T126A, 129(+A) |
| VP16 (UL48) | Q171R, T386A |
| VP13/14 (UL47) | V7A, E63D, E84G, 96–99(EEEE>Δ), Y144H, P432A |
| VP11/12 (UL46) | W108R, 301–304(AAVV>Δ), 508–509(GE>Δ), A532T |
| gB (UL27) | Y267H, R440Q, 506–507(SP>AA), P509Q |
| ICP18.5 (UL28) | A517V |
| UL32 | W266R, A289V |
| UL33 | 7(+G) |
| UL34 | D188G, Y221C |
| VP26 (UL35) | H65R |
| VP1/2 (UL36) c | T811A, L2152P, 2180(+EPTPAAKLAPPAPPPAKPA * )2, L2182T, H2219P, P2314L, K2449R, V2465A, T2666A, 2686(+PGDDVVPA), V2853F, E2876G |
| RR1 (UL39) | S95N, T294A |
| RR2 (UL40) | G266D |
| vhs (UL41) | M353V |
| UL42 | E331A |
| gC (UL44) | L14P, E43A, P156S |
| scaffold (UL26.5) | 120(+Q) |
| VP24 (UL26) | 365(+Q) |
| gH (UL22) | V59D, P438S, 481–482(EE>G) |
| UL21 | H37R, E355D, V375A |
| UL17 | G239D, S341G, P521L |
| UL15 | K404R |
| UL14 | Q18R |
| V18.8 (UL13) | A13V |
| AN (UL12) | 178(+GD), V474A |
| UL11 | Y49C |
| gM (UL10) | 59–60(TS>AP) |
| OBP (UL9) | D4P, 6–7(GG>RV) |
| UL8 | A52V, V509I |
| UL6 | 4(+AA) |
| UL5 | A2Δ |
| UL3.5 | H94Q, R193H |
| UL3 | T97Δ |
| UNG (UL2) | A286S |
| ICP4 (IE180) | Q92Δ, R195Δ, S234P, N296S, L319P, 371(P>LA), S752A, 854(+ESGSST), S954Δ, V1218A, H1390R |
| ICP22 (US1) | V31L, 239(+DEDEEEEE), 254(+DEDGLCEDE) |
| gG (US4) | 306–307(RG>HR) |
| gD (US6) | V69A, N82S |
| gI (US7), gE (US8), US9, US2 | Bartha deletion region |
Proteins are listed in order of occurrence along the genome (see Figure 3 for full list and functions). Table 2 lists proteins with AA differences from PRV Kaplan that are shared by PRV Bartha and Becker strains.
Bold indicates AA differences discussed in the text. Single AA residues changes are written in standard format, including the Kaplan reference strain AA, its position, and the AA residue found in Bartha's protein sequence, e.g. S100P. Insertions in PRV Bartha are indicated by the AA position in Kaplan followed by “+” and the new AAs, e.g. 100(+RR). Deletions are indicated by the symbol Δ. Sequential changes are combined and shown with the Kaplan strain AA positions first, followed by the relevant Kaplan AA residues, then “>”, and finally the new alternative AA residues, e.g. 100–102(RAR>EDA).
Subscript on parentheses in UL36 indicates multiple copies of a repeating unit.
*Final alanine (A) in this repeat unit changes to valine (V) in the second copy of the repeat unit.