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. 2011 Oct 13;7(10):e1002317. doi: 10.1371/journal.ppat.1002317

Figure 1. Western blot analysis of TcdC production by wild-type and complemented C. difficile strains.

Figure 1

(A) Qualitative analysis of TcdC production. VPI10463 is the positive control strain; VPI11186 is the negative control strain; M7404 is a wild-type Canadian BI/NAP1/027 strain; M7404(VC) is the wild-type strain carrying the shuttle plasmid pDLL4; M7404(tcdC +) is the wild-type strain carrying the tcdC expression plasmid pDLL17 and M7404(cured) is the M7404(tcdC +) strain cured of pDLL17. (B) Time course analysis of TcdC production by the positive control strain C. difficile VPI10463. Samples were taken at the indicated times shown in hours. 60 ng of purified recombinant his-tagged TcdC protein (rTcdC) was used as the positive reference sample. (C) Time course analysis of TcdC production by C. difficile strain M7404(tcdC +). Samples were taken at the indicated times shown in hours. 300 ng of purified recombinant his-tagged TcdC protein (rTcdC) was used as the positive reference sample. Western blots were performed with rabbit TcdC-specific antibodies. Size standards are shown (kDa).