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. 2011 Oct 13;7(10):e1002303. doi: 10.1371/journal.ppat.1002303

Figure 4. Co-localization of DDX6 with viral RNA and Gag near the MTOC.

Figure 4

HT1080 was co-transfected with pcPFV/gag-pum plus either pcmv-PUMHD(wt)_CitC (A) or pcmv-CitN_PUMHD(3794) (B), or co-transfected with pcPFV-wt (C) or pcPFV/gag-pum (D, E, & F) plus both expression vectors pcmv-PUMHD(wt)_CitC and pcmv-CitN_PUMHD(3794). At 32 h after transfection, cells were stained with rabbit anti-Gag (A to F) and mouse anti-DDX6 (C, E, & F) or mouse anti-γ-tubulin (D). Cells in Panel F were first treated with DDX6 siRNA before co-transfected with pcPFV/gag-pum and both expression vectors. Viral RNA containing PUMHD-binding sequences was viewed in green color (Left panel, D to F). Images were captured using DeltaVision microscopy.