FIGURE 2:

Appropriate levels of CIT-K are necessary for abscission. (A) Selected frames from time-lapse series of HeLa cells transfected with either Cherry or CIT-K-Cherry, using the same conditions as in Figure 1. Insets in the first frames represent red fluorescence of the imaged cells. During cytokinesis, CIT-K overexpression leads to both oscillatory movements (top) and delayed abscission (bottom). For full movies, see Movies S9 and S10. (B) Quantification of abscission time of HeLa cells expressing either Cherry or CIT-K-Cherry and not undergoing oscillatory movements during the time-lapse analysis. A total of 30 CIT-K-Cherry and 42 control cells were scored. Error bars represent SEM. Asterisks indicate that the difference is statistically significant using Student’s t test (p < 0.01).