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. 2011 Oct 15;22(20):3768–3778. doi: 10.1091/mbc.E10-12-0952

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© 2011 Gai et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 5: — CIT-K overexpression affects the localization of anillin and of active RhoA. (A) HeLa cells were transfected with either Cherry or Cherry-CIT-K. Interphase cells were analyzed by immunofluorescence for localization of the endogenous anillin or active RhoA. (B) Selected frames from time-lapse series of HeLa cells undergoing cytokinesis. HeLa cells were cotransfected with GFP-ceRhoA and CIT-K-Cherry and observed 30 h posttransfection by time-lapse video microscopy. For full movie, see Movie S11. (C) HeLa cells in late cytokinesis, expressing either Cherry or CIT-K-Cherry, were analyzed for the localization of active RhoA by immunofluorescence 30 h posttransfection. Nuclei were counterstained with DAPI.