FIGURE 6:

CIT-K or anillin overexpression delays abscission. (A) HeLa cells expressing either Cherry or CKA-Cherry were synchronized with the thymidine/nocodazole method, treated with vehicle or with C3 transferase protein 2 h after release, and analyzed after one additional hour. Slides were then scored for the fraction of telophase cells, as determined by DAPI and α-tubulin staining and fluorescence microscopy. In every case, error bars represent SEM. Asterisks indicate that the difference is statistically significant using Student’s t test (*p < 0.05; **p < 0.01; ***p < 0.001). (B) HeLa cells were transfected with GFP, anillin (ANLN)-FL, or anillin-ΔNLS and analyzed as in (A).