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. 2011 Oct 15;22(20):3801–3811. doi: 10.1091/mbc.E11-01-0057

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© 2011 Kelly and Nurse. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 4: — Both pathways can localize independently. (A) Rga4-3×GFP does not require Scd1 or Scd2 for localization to cell sides. (B) Neither Scd1-3×GFP nor Scd2-3×GFP is dependent on Rga4 for localization to cell tips. (C) Scd1-3×GFP is lost from the cell tips in scd2Δ. (D) Scd2-3×GFP is mislocalized in scd1Δ. Images for all are representative, deconvolved, single focal planes with inverted LUTs. Scale bars: 5 μm. Unless marked, cells were in exponential growth at the time of imaging. scd1Δ is also shown after growth for 5 h in HU to elongate the cells.