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. 2011 Oct 15;22(20):3812–3825. doi: 10.1091/mbc.E11-04-0328

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© 2011 He et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 2: — SGK3 rapidly activates NHE3 in response to Dex. (A) PS120/NHE3V cells were stably transfected with HA-SGK1, HA-SGK2, and HA-SGK3, and the expression levels of SGKs were determined by immunoblotting using an anti-HA antibody. Western blot of β-actin was used as loading control. PS120/NHE3V cells expressing (B) HA-SGK1, (C) HA-SGK2, or (D) HA-SGK3 were treated with 1 μM Dex for t = 0, 15 min, 4 h, or 24 h, followed by determination of NHE3 activity. The rates of pH recovery at pH_i 6.7 are shown. n = 8. *, p < 0.01 compared with the untreated control.