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. 2011 Oct 15;22(20):3812–3825. doi: 10.1091/mbc.E11-04-0328

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© 2011 He et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 6: — SGK3 colocalizes with NHE3 in recycling endosomes. (A) Cellular distribution of HA-tagged SGK1-3 (green) and NHE3V (red) in PS120 cells was determined by indirect immunofluorescence with anti-HA and anti-VSVG antibodies, respectively. The inset shows a magnified image of the boxed area. (B) Cellular localization of HA-tagged SGK1-3 (green) and Rab5 (red) in PS120 cells was determined with anti-HA and anti-Rab5 antibodies, respectively. (C) PS120/NHE3V cells were transfected with FLAG-tagged SGK1 or SGK2 and GFP-Rab11. The colocalization of SGK1 or SGK2 with Rab11 was determined with anti-FLAG antibody. (D) PS120/NHE3V cells were transfected with FLAG-SGK3 and GFP-Rab11. The colocalization of SGK3 (red) and NHE3V (blue) with GFP-Rab11 (green) was determined with anti-FLAG and anti-VSVG antibodies, respectively. Scale bar: 10 μm.