Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2011 Oct 15;22(20):3812–3825. doi: 10.1091/mbc.E11-04-0328

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2011 He et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

“ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell Biology.

PMC Copyright notice

FIGURE 7: — Acute regulation of NHE3 by SGK3 is dependent on the endosomal localization of SGK3. (A) PS120/NHE3V cells were expressed with HA-SGK3 or HA-SGK3-R90A, and the expression levels of SGK3 and SGK3-R90A in PS120/NHE3V cells were examined with anti-HA antibody. (B) Cellular distribution of SGK3 (green) or SGK3-R90A (green) with NHE3 (red) in PS120 cells was examined with anti-HA and anti-VSVG antibodies, respectively. Scale bar: 10 μm. (C) PS120/NHE3V/HA-SGK3-R90A cells were treated with Dex for t = 0, 15 min, 4 h, or 24 h, and SGK3-R90A protein was purified by immunoprecipitation for kinase assay. The kinase activities of SGK3-R90A are shown relative to the basal activity. n = 3. (D) PS120/NHE3V/HA-SGK3-R90A cells were treated with 1 μM Dex for t = 0, 15 min, or 4 h, followed by measurement of NHE3 activity. The rates of pH recovery at pH_i 6.7 are shown. n = 6. *, p < 0.01 and **, p < 0.05, compared with the untreated control.