FIGURE 2:

Effect of the PKA inhibitors H-89 and KT5720 on the prevention by Glu and Sal of E17G-induced impairment of cVA of CLF (A) and GS-MF (B). Couplets pretreated for 15 min with H-89 (200 nM), KT5720 (50 nM), or vehicle (DMSO) in controls were subsequently exposed to Glu or Sal for a further 15-min period and then exposed to E17G (50 μM) for an additional 20-min period. cVAs of CLF and GS-MF were calculated as the percentage of couplets displaying visible fluorescence in their canalicular vacuoles from a total of at least 200 couplets per preparation, referred to control cVA values. Control cVA values were 76 ± 1% for CLF and 76 ± 2% for GS-MF. Data are expressed as mean ± SEM (n = 3). ^aSignificantly different from control (p < 0.05). ^bSignificantly different from E17G and control (p < 0.05).