FIGURE 7:
Estimation of MEK activation by salbutamol and 8-CPT-2´-O-Me-cAMP. Isolated rat hepatocytes were incubated with Glu (0.1 μM), Sal (1 μM), and 8-CPT-2´-O-Me-cAMP (8-CPT-cAMP, 50 μM, positive control). MEK activity was determined by immunoblots, using an antibody against phosphorylated MEK1/2. A band at 45 kDa was detected. Differences in sample loading were corrected by the densitometric signal of the corresponding actin band. The ratio of each pMEK/actin band density was compared with the ratio of control bands (100%). Data are expressed as mean ± SEM (n = 3). aSignificantly different from control (p < 0.05).