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. Author manuscript; available in PMC: 2012 Nov 1.
Published in final edited form as: J Neurochem. 2011 Sep 23;119(3):544–554. doi: 10.1111/j.1471-4159.2011.07457.x

Figure 4. Delayed cerebellar development and deficient Purkinje cell maturation in Gβ5-homozygous knockout mice.

Figure 4

Analysis of Pcp2-GFP hemizygous Gβ5 KO (A, C, E, G) and Pcp2-GFP hemizygous Gβ5 wild-type (B, D, F, H) littermate mice. (A, B) Merged DAPI (blue) and Pcp2-GFP reporter histofluorescence (green) images from cerebellar sections harvested from seven-day old Gβ5 homozygous KO and wild-type littermates (50X). (C-H) Laser confocal analysis of Pcp2-GFP reporter histofluorescence in cerebellar sections from 10-day old mice comparing the degree of dendritic arborization in Gβ5 homozygous KO mice and their wild-type littermates. Magnification in C, D, 20 X; in E, F, 40 X. See also supplemental videos 3 and 4, corresponding to images G and H, showing 3-D images of the corresponding neurons reconstructed from a stack of confocal images acquired at 0.17 μm step size, as described in supplementary online materials and methods. Results in A-F are representative of results from three wild-type and Gnb5 KO sibling pairs.