Fig. 8.

Pax8 is not expressed in human pancreatic neuroendocrine tumors. A Pax8 or Pax6 immunostaining in two different pancreatic neuroendocrine tumors using polyclonal (a, d) or monoclonal (c, f) Pax8 antibody, or Pax6 antibody (b, e) reveals the lack of Pax8 staining when Pax8-specific monoclonal antibody is used. Of note, the lower panel tumor is also negative for Pax6. B Pax8 or Pax6 immunostaining in human kidney and C liver using polyclonal (g, j) or monoclonal (i, l) Pax8 antibody, or Pax6 antibody (h, k). An overlap in the immunostaining signal between the two Pax8 antibodies in kidney tubule epithelia, indicates the expression of Pax8 in this tissue. No nuclear immunostaining for Pax6 was detected in kidney. Pax8 or Pax6 antibodies revealed no signals in the liver