Table 6.
PsaA/PsaB residue pairs responsible for ΔEm (= Em(PA) – Em(PB), see Fig. 2) (mV)
| PsaA |
PsaB |
|||||
|---|---|---|---|---|---|---|
| Residue | Em(PA) | Em(PB) | Residue | Em(PA) | Em(PB) | ΔEm |
| ΔEm increasing pairs (i.e., pairs that contribute to accumulation of PB•+) | ||||||
| Arg-A750 | 65 | 36 | Ser-B734 | 9 | 21 | 17 |
| — | — | — | Asp-B92 | −15 | −24 | 9 |
Contribution to ΔEm was obtained as [influence of PsaA on Em(PA)] + [influence of PsaB on Em(PA)] – [influence of PsaA on Em(PB)] – [influence of PsaB on Em(PB)]. PsaA/PsaB residue pairs in each line were generated from the protein sequence alignment performed with the CLUSTAL program (31) (Fig. 4).