Table 2.
Melting temperatures (Tm) determined by UV absorption at 295 nm
| Sequence name | Tm | ΔTm (°C) | Average ΔTm (°C)∗ |
|---|---|---|---|
| Unmodified | 57.0 | — | — |
| Br-3 | 59.5 | 2.5 | |
| Br-9 | 63.9 | 6.9 | |
| Br-15† | 62.3 | 5.3 | 5.2 ± 2.7 |
| Br-16 | 62.2 | 5.2 | |
| Br-21† | 63.2 | 6.2 | |
| Br-22 | 55.3 | −1.7 | — |
| Br-9-22 | 60.7 | 3.7 | — |
| Omet-3 | 59.6 | 2.6 | |
| Omet-9 | 60.5 | 3.5 | |
| Omet-15 | 58.9 | 1.9 | 3.1 ± 1.4 |
| Omet-16 | 60.1 | 3.1 | |
| Omet-21† | 61.5 | 4.5 | |
| Omet-22 | 59.0 | 2.0 | — |
| Br-9-Omet-22 | 64.6 | 7.6 | — |
| Am-3 | 57.5 | 0.5 | |
| Am-9 | 58.7 | 1.7 | |
| Am-15 | 57.0 | 0.0 | 1.1 ± 1.1 |
| Am-16 | 58.4 | 1.4 | |
| Am-21 | 59.1 | 2.1 | |
| Am-22 | 54.0 | −3.0 | — |
| Br-9-Am-22 | 59.3 | 2.3 | — |
| Oxo-3 | 47.2 | −9.8 | — |
| Oxo-9 | 38.7 | −18.3 | — |
| Oxo-15 | 38.5 | −18.5 | — |
∗
Average Tm was calculated considering only the syn position substitutions for each modification type. Values are represented as the mean ± maximum deviation.
†
Samples with a small additional structural transition at low temperature in their denaturing profile.