Figure 7.
Effect of plasma and cell surface lipids on the sensitivity to local TF density during coagulation. (A) Thrombin production by prothrombinase assembled on PL, films with fibroblasts, or films with immobilized TFs. Shown are the mean ± SD, n = 8÷16. (B) fXa production by intrinsic tenase assembled on either PL, films with fibroblasts, or films with immobilized TFs. Shown are the means ± SD, n = 4÷6. (C) Effect of PL (0 and 10 μM) and fVIIa (0 and 10 nM) on the clotting initiation time. Clot formation was initiated by films with fibroblasts or immobilized TFs, both with an average surface density of 8 pmol/m2. Control films are films without TFs. Shown are the means ± SD, n = 3÷7. Asterisks show a significant difference, and double asterisks show a lack thereof, as calculated using an unpaired, two-sample t-test at p = 0.05.