Folding kinetics of GB1. Top panel: Chevron plots measured from pH 2.0 to pH 5.5 at 25°C (blue, pH 2.0; green, pH 2.5; violet, pH 3.0; red, pH 3.5; magenta, pH 4.0; cyan, pH 4.5; yellow, pH 5.0; orange, pH 5.5). Bottom panel: Chevron plots measured from pH 5.5 to pH 9.6 at 25°C (orange, pH 5.5; blue, pH 6.0; black, pH 6.5; cyan, pH 7.0; magenta, pH 7.5; violet, pH 8.0; yellow, pH 8.5; red, pH 9.0; green, pH 9.6). Lines are the best global fit to a three-state equation with shared m-values. If data at [GdnHCl] > 5.5 M were to be ignored, a quasilinear unfolding arm with an apparent change in unfolding m-values would be seen.