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Unfolding rate constants versus pH. Logarithm of the calculated unfolding rate constants from the native state N to the first transition state ku(TS1), and from the native state to the second transition state ku(TS2) as a function of pH. The lines are the best fit to a model involving the protonation of two groups. In both cases, we obtained approximately the same pKa of ∼4 and ∼8.
