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. 2011 Jul 22;62(14):4805–4817. doi: 10.1093/jxb/err120

Fig. 6.

Fig. 6.

Transient expression of PwHAP5 or PwFKBP12 in P. wilsonii pollen tube. The overexpression vectors PwHAP5 or PwFKBP12 fused to CHERRY or GFP, respectively, were constructed and expressed transiently alone or used to co-transform P. wilsonii pollen by microprojectile bombardment. The pollen-specific expression promoter Lat52 was used to drive the expression of PwHAP5 or PwFKBP12 in pollen tube. Microscopic analysis of pollen tube expression was performed 24 h after gene transfer of PwFKBP12 and PwHAP5 in pollen. Transient expression of PwHAP5 alone affects P. wilsonii pollen tube growth orientation, whereas both transient expression of PwFKBP12 and co-expression of PwHAP5 and PwFKBP12 do not. Left panel: transmitted light images. Right panel: fluorescence images. The yellow fluorescence is the overlay of GFP and CHERRY proteins. The data were obtained from three independent experiments, and each condition was tested three times. Bars, 20 μm.